The preimaginal stages of 14 species of Chrysidini—three postdefecating larvae (prepupae), nine mature larvae, two second-stage larvae, and nine first-stage larvae—are described. After analyzing for 33 selected characters, the character states observed in the mature larvae studied, several apomorphies are described that could characterize the groups Bethylidae, Chrysididae, Chrysidinae, and Chrysidini, as well as the genera Caenochrysis, Chrysis, Exochrysis, and Trichrysis. Second-stage larvae are very similar to mature larvae, those of the Chrysidini and Elampini being differentiated by the number of mandibular teeth. First-stage larvae display several morphological particularities derived from their cleptoparasitoid or parasitoid nature, emphasis being placed on the long antennae, the degree of development of setae on the labrum, the ventral position of the maxillary and labial palpi, the strong development of the mandibles, and the morphology of the last abdominal segment. For first-stage larvae, after analyzing 20 selected characters, different apomorphies that could characterize the genera Caeonochrysis, Chrysura, Pseudospinolia, and Trichrysis are discussed.